Generic selectors
Exact matches only
Search in title
Search in content
Search in posts
Search in pages
Filter by Categories
CBAB 19(4) 2019
Editions
CBAB 19(3) 2019
Uncategorized
CBAB 19(2) 2019
CBAB 19(1) 2019
CBAB 18(4) 2018
CBAB 18(3) 2018
CBAB 18(2) 2018
CBAB 18(1) 2018
CBAB 17(4) 2017
CBAB 17(3) 2017
CBAB 17(2) 2017
CBAB 17(1) 2017
CBAB 16(4) 2016
CBAB 16(3) 2016
CBAB 16(2) 2016
CBAB 16(1) 2016
CBAB 15(4) 2015
CBAB 15(3) 2015
CBAB 15(2) 2015
CBAB 15(1) 2015
CBAB 14(4) 2014
CBAB 14(3) 2014
CBAB 14(2) 2014
CBAB 14(1) 2014
CBAB 13(4) 2013
CBAB 13(3) 2013
CBAB 13(2) 2013
CBAB 13(1) 2013
CBAB 12(S2) 2012 Special Edition
CBAB 12(4) 2012
CBAB 12(3) 2012
CBAB 12(2) 2012
CBAB 12(1) 2012
CBAB 11(S1) 2011 Special Edition
CBAB 11(4) 2011
CBAB 11(3) 2011
CBAB 11(2) 2011
CBAB 11(1) 2011
CBAB 10(4) 2010
CBAB 10(3) 2010
CBAB 10(2) 2010
CBAB 10(1) 2010
CBAB 09(4) 2009
CBAB 09(3) 2009
CBAB 09(2) 2009
CBAB 09(1) 2009
CBAB 08(4) 2008
CBAB 08(3) 2008
CBAB 08(2) 2008
CBAB 08(1) 2008
CBAB 07(4) 2007
CBAB 07(3) 2007
CBAB 07(2) 2007
CBAB 07(1) 2007
CBAB 06(4) 2006
CBAB 06(3) 2006
CBAB 06(2) 2006
CBAB 06(1) 2006
CBAB 05(4) 2005
CBAB 05(3) 2005
CBAB 05(2) 2005
CBAB 05(1) 2005
CBAB 04(4) 2004
CBAB 04(3) 2004
CBAB 04(1) 2004
CBAB 03(4) 2003
CBAB 03(3) 2003
CBAB 03(2) 2003
CBAB 03(1) 2003
CBAB 02(4) 2002
CBAB 02(3) 2002
CBAB 02(2) 2002
CBAB 02(1) 2002
CBAB 01 (4) 2001
CBAB 01 (3) 2001
CBAB 01 (2) 2001
CBAB 01 (1) 2001
CBAB News
+55 31 3612-4490
cbabjournal@gmail.com
Close

Wendell Jacinto Pereira, Priscila Zaczuk Bassinello, Claudio Brondani and Rosana Pereira Vianello

Abstract: An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris.

Get the article here. (PDF)

Add Your Comment

Your email address will not be published. Required fields are marked *