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Carla Dubiela, Tatiane Dalla Nora Montecelli, Fabiane Lazzari, Eliezer Rodrigues de Souto and Ivan Schuster

Abstract: This work aimed to develop and validate individual SNP molecular
markers previously identified in a genetic association study of resistance to Meloidogyne
incognita in soybean using a microarray panel. The markers identified
in the array were converted in single TaqMan® markers. The single markers were
used to create an SNP genotyping protocol and establish a marker-assisted selection
(MAS) routine associated with resistance to M. incognita in soybean. Out of
the eight TaqMan® assays tested, three were validated for use in MAS. The MAS
protocol developed in this study uses sequential selection. Initially, molecular
markers are used to identify susceptible plants; subsequently, the phenotypic
evaluation of plants expressing resistance genotype for the markers is carried
out, resulting in the accurate identification of resistant plants. The accuracy
of this approach for MAS sequential for M. incognita varied from 94 to 96%.

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