Generic selectors
Exact matches only
Search in title
Search in content
Search in posts
Search in pages
Filter by Categories
CBAB 20(2) 2020
Editions
CBAB 20(1) 2020
CBAB 19(4) 2019
Uncategorized
CBAB 19(3) 2019
CBAB 19(2) 2019
CBAB 19(1) 2019
CBAB 18(4) 2018
CBAB 18(3) 2018
CBAB 18(2) 2018
CBAB 18(1) 2018
CBAB News
CBAB 17(4) 2017
CBAB 17(3) 2017
CBAB 17(2) 2017
CBAB 17(1) 2017
CBAB 16(4) 2016
CBAB 16(3) 2016
CBAB 16(2) 2016
CBAB 16(1) 2016
CBAB 15(4) 2015
CBAB 15(3) 2015
CBAB 15(2) 2015
CBAB 15(1) 2015
CBAB 14(4) 2014
CBAB 14(3) 2014
CBAB 14(2) 2014
CBAB 14(1) 2014
CBAB 13(4) 2013
CBAB 13(3) 2013
CBAB 13(2) 2013
CBAB 13(1) 2013
CBAB 12(S2) 2012 Special Edition
CBAB 12(4) 2012
CBAB 12(3) 2012
CBAB 12(2) 2012
CBAB 12(1) 2012
CBAB 11(S1) 2011 Special Edition
CBAB 11(4) 2011
CBAB 11(3) 2011
CBAB 11(2) 2011
CBAB 11(1) 2011
CBAB 10(4) 2010
CBAB 10(3) 2010
CBAB 10(2) 2010
CBAB 10(1) 2010
CBAB 09(4) 2009
CBAB 09(3) 2009
CBAB 09(2) 2009
CBAB 09(1) 2009
CBAB 08(4) 2008
CBAB 08(3) 2008
CBAB 08(2) 2008
CBAB 08(1) 2008
CBAB 07(4) 2007
CBAB 07(3) 2007
CBAB 07(2) 2007
CBAB 07(1) 2007
CBAB 06(4) 2006
CBAB 06(3) 2006
CBAB 06(2) 2006
CBAB 06(1) 2006
CBAB 05(4) 2005
CBAB 05(3) 2005
CBAB 05(2) 2005
CBAB 05(1) 2005
CBAB 04(4) 2004
CBAB 04(3) 2004
CBAB 04(1) 2004
CBAB 03(4) 2003
CBAB 03(3) 2003
CBAB 03(2) 2003
CBAB 03(1) 2003
CBAB 02(4) 2002
CBAB 02(3) 2002
CBAB 02(2) 2002
CBAB 02(1) 2002
CBAB 01 (4) 2001
CBAB 01 (3) 2001
CBAB 01 (2) 2001
CBAB 01 (1) 2001
+55 31 3612-4490
cbabjournal@gmail.com
Close

Simara da Silva Lopes, Jéssica Fabiane da Veiga Costa, Beatriz de Almeida Barros, Andrea Almeida Carneiro and Sylvia Morais de Sousa

Abstract: Determining the copy number is important because it can greatly
influence the expression level and genetic stability of transgenes. This study
aimed to establish a methodology that can estimate the transgene copy number
in tobacco (Nicotiana tabacum) using the Auxin independent1 (Axi1) gene as
an endogenous control. Tobacco c.v. Petit Havana plants were transformed via
Agrobacterium tumefaciens with a pMCG1005 vector that contained Bar as a
selective gene. Oligonucleotide efficiency was determined and a qPCR using the
2-ΔCt and 2-ΔΔCt methods was performed. Bar was the target gene and Axi1 was
the endogenous control in five transgenic tobacco events. The results showed
that the Axi1 gene was donated by the maternal parent N. sylvestris when interspecific
hybridization occurred between N. sylvestris and N. tomentosiformis.
The copy number results agreed with the segregation ratios for the Bar gene
in T1 plants, which confirmed that Axi1 is a single copy gene that can be used
as an endogenous control.

Get the article here. (PDF)

Add Your Comment

Your email address will not be published. Required fields are marked *