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Thyana Lays Brancher, Maraisa Crestani Hawerroth , Marcus Vinícius Kvitschal , Danielle Caroline Manenti and Altamir Frederico Guidolin

ARTICLE – Self-incompatibility alleles in important genotypes for apple breeding in Brazil

Abstract: The objective of this study was to identify self-incompatibility (S) alleles
of advanced breeding selections of apple (Malus × domestica Borkh.). The Salleles of 42 apple genotypes were analyzed by markers using allele-specific PCR
amplification and amplicons digested with restriction endonucleases. Among
the screened genotypes were cultivars, advanced selections, and accessions
of the Apple Germplasm Bank of Epagri (Caçador, Santa Catarina, Brazil). Two
S-alleles were identified in 36 genotypes, and only one S-allele was determined
in the other six genotypes. In all, eleven S-alleles were identified among all
the genotypes evaluated. The S3
and S5
alleles were most frequent (30.2% and
18.6%, respectively). The identification of S-alleles using molecular markers
in important apple tree genotypes is useful for determination of compatible
parents for breeding programs.

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