Use of Markers as a Tool to Investigate the Presence of Disease Resistance Genes in Common Bean Cultivars
Ana Lilia Alzate-Marin; Marcia Regina Costa; Aloisio Sartorato; Carlos A. Rava; Everaldo Gonçalves de Barros and Maurilio Alves Moreira
The molecular marker-assisted selection has a great potential as a breeding tool in common beans. The identification of RAPD markers allows association (pyramiding) of genes for disease resistance or the identification of high potential cultivars with different resistance genes that were not previously identified due to limitations in the screening methodology. In this study previously identified molecular markers were used as a tool to investigate the presence of disease resistant genes as well as to identify pyramided genes that are present in the lines tested in the elite lines trials coordinated by Embrapa Rice and Beans. Fourteen out of 21 elite lines possessed different molecular markers linked to disease resistant genes for anthracnose, angular leaf spot, rust and bean golden mosaic virus. Lines FEB 163 (5) and TB 94-01 (8) presented seven molecular markers linked to different resistance genes. It was not possible to detect the presence of any known sources of disease resistance genes, based in the pedigree of lines LM 93204319 (12), LM 93204328 (13), LM 93203246 (18) and PR 93201472 (21).