Crossing and diagnostic methods of cubiu hybrid plants based on genetic markers
Jean Rodrigo Pizzinato, Adilson Ricken Schuelter, Antonio Teixeira do Amaral Júnior, Andressa Camilo de Souza Rocha, Jaqueline Manzatti da Siva, Cátia Raquel Volkweis, Mateus Brusco de Freitas, Fabiano Ricardo, and Diogo Oswaldo Schwantes
The objective of this study was to evaluate the effect of different artificial cross pollination techniques in cubiu (Solanum sessiliflorum) for breeding purposes and genetic control studies of economically important traits. The varieties Santa Luzia and Thaís, with and without protection with organza cloth, were used in natural crosses and crosses with emasculation. The methods were evaluated for pollination efficiency and fruit traits, namely: weight, length, width, length/ width ratio and mean number of seeds. RAPD analysis indicated that all progenies were derived from the controlled crosses, which confirmed the pollination efficiency of the methods applied. The pollination efficiency varied from 4.57% to 8.37% among the crosses. The use of emasculation without organza cover was statistically best for fruit yield. The variety Santa Luzia is recommended as female parent for hybridization. Crossing seems to be the predominant mechanism of reproduction in this species.