Molecular analysis of the seedlessness character in grape using RAPD markers
Júlio César de Lima, Adriana Crippa, Lucas da Ressurreição Garrido, Umberto Almeida Camargo, and Paulo Ricardo Dias de Oliveira
Forty-eight polymorphic RAPD primers were used to study the genetic similarity of eight grapevine (Vitis spp.) accessions: Itália, Seyve Villard 12327, Seyve Villard 12375, Crimson Seedless, CG 87746, A 1976, Gota de Ouro and CNPUV 154-27. Gota de Ouro, the only Vitis labrusca cultivar, was the most distant cultivar from the others. The population CNPUV 154 (Seyve Villard 12327 x CG 87746) was evaluated for fresh and dry seed weight. Four phenotypic classes were considered for dry matter percentage (DM%): 1 (DM > 55), 2 (45 < DM < 55), 3 (35 < DM < 45) and 4 (DM < 35). The observed frequencies for the classes 1, 2, 3, and 4 were 37, 27, 53, and 9 individuals, respectively. Of the 500 RAPD screened primers, 111 were selected as polymorphic. According to BSA, markers generated by OPK02, OPM16, OPS03, and OPU13 primers could be related to the region that controls seedlessness. Get the article here. (PDF)