Genetic diversity of common bean genotypes of Carioca commercial group using RAPD markers
Edwin Camacho Palomino, Edson Seizo Mori, Léo Zimback, Evandro Vagner Tambarussi, and Cristiano Bueno de Moraes
The objective of this research was to characterize the genetic diversity of bean genotypes (Phaseolus vulgaris L.), from Carioca commercial group, utilizing RAPD markers to discriminate and to support breeders to choose genetic material and to develop breeding methods to use for obtaining new cultivars with Carioca type seeds. Twenty-eight genotypes, fifteen cultivars, and thirteen lines were evaluated. The genetic distances were achieved utilizing the Euclidian’s method modified by Roger’s (1972) and the grouping analysis were by UPGMA method, showed by a dendogram. Through 89 RAPD loci, obtained from 12 primers, 65.17% were polymorphics. We have verified that the RAPD markers were efficient in discrimination and grouping of genotypes, showing to have large genetic variability. Although Carioca cultivar has a common ancestor from all studied material, it has shown low similarity with genotypes derived from it, corroborating to exist sufficient genetic variability for production of new cultivars, maintaining the original coloration of seeds.