Genetic analysis and gene mapping of the purple glume tip trait in rice (Oryza sativa)
Gang Jin, Zhongquan Cai, Yanxi Chen, Ying Ling, Liping Wang and Deyuan Mo
Abstract: The purple glume-tip is an essential morphological marker for selective rice breeding, aiding in assisted selection and variety purification. However, the inheritance of purple glume-tip in japonica rice landrace Donglan Black Rice (DBR) has not yet been explored deeply. The F2 and F4 populations were constructed from crossing between Huazhan with colorless glume-tip and DBR to identify the associated genomic region(s). Genetic analysis displayed two highly comparable and perplexing phenotypes in the purple glume-tip of rice. Two significant genes with recessive epistasis predominantly regulated the two phenotypes. The two target gene loci were located in the intervals of 5315163–5316875 bp on chr6 and 27915598-27939357 bp on chr4, respectively, where reported genes associated with the purple color trait in rice, Os06g0205100 and Os04g0557500, are present. The two genes may be potential target genes. However, the role of Os04g0557500 in the glume-tip coloration remains unreported.