Colchicine treatments in anther culture of soybean
Eliane Kaltchuk Santos, Ana Paula de Moraes, Elsa Mundstock and Maria Helena Bodanese-Zanettini
Abstract: The effect of colchicine on the symmetrical division and embryo induction in cultured anthers of soybean was investigated. The anthers were incubated in various concentrations of colchicinfeor 24 or 72 hours. The in vitro-incubated anthers were cytologically analyzed throughout the first 15 days of culture. The study showed that colchicine did not have a significant effect on the symmetrical division or multinucleated pollen formation. Embryo induction was also not affected by colchicine. Embryos showed histodifferentiation and were classified in various morphological classes that resembled somatic embryos obtained from immature cotyledons.