Development and validation of SSR markers for Coffea arabica L.
Robson Fernando Missio; Eveline Teixeira Caixeta; Eunize Maciel Zambolim; Laércio Zambolim and Ney Sussumu Sakiyama
Abstract: With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n’ (AC/GT)n’ (AAG/CTT)n’ and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica.