KASP-Assisted pyramiding of Null KTI and Null Lox2 with YMD resistance in Indian soybean
Kamalpreet Singh Mandahal, Karmvir Singh Garcha, Harmandeep Kaur, Asmita Sirari, Satinder Kaur, Vineet Kumar and Balwinder Singh Gill
Abstract: The current study was planned to pyramid Yellow Mosaic Disease (YMD) resistance with null KTI and null Lox2 in soybean, with a purview to create food grade soybean genotypes which can sustain under heavy disease pressure of North Indian plains. Hence, a cross was made between NRCSL 3 (R) x NRC 142 (S, null KTI + null Lox2). F2 and subsequent F5, was screened using newly developed functional KASP markers for both traits. The markers were validated on a panel of F1 hybrids (derived from 3 different crosses) as well as wild type and double null parents. Using the markers, a total of 15 double null lines i.e., null KTI + null Lox2; having YMD resistance were identified. Two of these outyielded the best check and parent NRCSL 3 in terms of economic yield. This is the first report of pyramiding of YMD resistance with null KTI and null Lox2.