Genetic improvement of Chakhao rice by gene stacking of high-yielding and durable blast-resistant traits
Monalisa Nameirakpam, Sanatombi Y., Umakanta Ngangkham, Basanta Singh T, Rishikanta Singh Kh, Philanim W.S., Awadhesh Kumar, E Lamalakshmi, Sarika Konsam, Sonia Chongtham, Ratankumar Singh Akoijam, Anand Meetei W., Bijen kumar Yengkhom, Tania Chongtham, Umananda A., Diviya Th. and Ramgopal Laha
Abstract: Chakhao is a popular pigmented black rice variety with remarkably high anthocyanin content. Due to its susceptibility to various biotic and abiotic stresses, its production has remained low. Here, two genes, Pi54 blast-resistant and OsSPL14, high-yielding genes of rice, were pyramided in a Chakhao landrace by crossing with CR Dhan 307. From a total of 147 F4 lines developed, 32 were identified with the positive alleles of both genes, of which 16 were black grain coloured, and their total anthocyanin content ranged from 30.19±2.19 to 240.31±2.62 mg 100 g-1 dried weight. Among these, ChM 68 F4 line had the highest anthocyanin content (240.31 ± 2.62 mg 100 g-1 of powdered grain)
with more 118.96 mg 100 g-1 anthocyanin than the recipient parent, CHK13 (121.35±3.32 mg 100 g-1), making it a promising line to be released as a highy-ielding and durable blast-resistant Chakhao variety in Manipur.