The 34-bp deletion effectively differentiates the monoecious phenotype in cucumber
Linh T.T. Le
Abstract: The sex phenotype in cucumbers, determined by the F locus, is crucial for crop productivity. Identifying markers for this trait aids in selecting gynoecious varieties for higher yields, but existing molecular markers show low correlation with the gynoecious trait. This challenge can be addressed by indirectly selecting target lines and eliminating monoecious genotypes through accurate screening. This study seeks to develop a molecular marker closely linked to the F locus to differentiate monoecious cucumber phenotypes. Genetic analysis of a 1,000 kb region around the F locus in both gynoecious and monoecious lines revealed two polymorphisms: a 34-base pair deletion (34Del) and a 16-base pair insertion (16In). Only the 34Del polymorphism consistently co-segregated with the monoecious phenotype, effectively distinguishing homozygous from heterozygous monoecious lines. Therefore, the 34Del polymorphism can serve as a reliable marker for use in cucumber breeding programs to improve yield.