Identification of brown rust resistance in the field and detection of the Bru1 gene in sugarcane varieties
Rong-Yue Zhang, Hong-Li Shan, Kun Yang, Xiao-Yan Wang, Xiao-Yan Cang, Chang-Mi Wang, Jiong Yin, Zhi-Ming Luo, Wen-Feng Li and Ying-Kun Huang
Abstract: The aim of this study was to assess brown rust resistance in 60 new sugarcane varieties bred by the China Sugarcane System in recent years and in 34 major varieties cultivated in sugarcane growing areas in China. The resistance of these sugarcane varieties to brown rust was investigated in the field, and molecular markers were used to detect the brown rust-resistance gene Bru1. The results of the field survey showed that 66 (70.21%) of the 94 sugarcane varieties were highly resistant to moderately resistant, and 28 (29.79%) were susceptible to highly susceptible. The Bru1 gene was detected in 54 (57.45%) of the 94 sugarcane varieties. Seven highly resistant varieties and five resistant varieties did not carry Bru1, suggesting that they carry other genes associated with brown rust resistance. This study provides a scientific basis and identifies disease-resistant germplasm for selection of plants for sugarcane production.