Comparison between doubled haploid lines and lines obtained via the bulk method in tobacco
Roxane do Carmo Lemos, José Maria Vilella Pádua, Adriano Teodoro Bruzi, Vando Braz de Oliveira and Magno Antonio Patto Ramalho
Abstract: This study aimed to compare recombinant inbred lines (RIL) obtained via the bulk method with doubled haploid lines (DHL). For comparison, 190 DHL and 194 RIL were evaluated simultaneously at two different locations. Phenotypic and genetic parameters were estimated for the traits green leaf yield (GLY) and alkaloid content (ALK). The results of the RIL and DHL evaluations were similar for ALK. Conversely, estimates of genetic variation and heritability in the DHL were higher than those in the RIL for GLY. However, the mean estimate of the RIL was 13.3% higher than that of the DHL and thus, the annual gain with selection was higher for the RIL. The use of DHL in a breeding program program will be efficient in comparison to RIL if a large number of lines are obtained, which must undergo a preliminary selection before the most intensive evaluation to identify the lines to be recombined.