In Vitro Adventitious Shoot Regeneration from Sweet Orange Using Thin Epicotyl Sections
João Carlos Bespalhok Filho; Adilson K. Kobayashi; Luiz F. P. Pereira; Zuleide Hissano and
Luiz G. E. Vieira
A system for in vitro plant regeneration from thin transversal epicotyl sections of sweet orange (Citrus sinenis L Osbeck cvs Pera, Valencia and Folha Murcha) was developed. Thin section explants (1-2 mm) from seedlings produced adventitious shoot buds on MS medium supplemented with BA (0.5-5 mM). Optimal shoot induction was achieved on 2-5 mM BA. Explants with buds were transferred to elongation media in order to improve the recovery of normal shoots. Higher number of elongated shoots was obtained on medium with 0.5 mM BA. Elongated shoots were rooted on half strength MS medium without growth regulators and transferred to soil for acclimatization. The advantages of this system for transformation are discussed.