Validation of a RAPD marker linked to the anthracnose resistant gene Co-5 in the common bean
Ana Lilia Alzate-Marin; Klever Marcio Arruda; Everaldo Gonçalves de Barros and Maurilio Alves Moreira
Cultivar TU, one of the differential cultivars for anthracnose, carries the resistance gene Co-5 that expresses resistance to a wide range of pathotypes in Brazil. The main goals of this study were to analyze the inheritance of resistance to anthracnose in the cultivar TU in segregating populations derived from crosses with cultivar Rudá, and to validate the random amplified polymorphic DNA (RAPD) marker OPB03450C, previously identified as being linked to resistance gene Co-5 in cultivar Selection 1360. Results revealed that one single gene present in cultivar TU, Co-5 is responsible for resistance, and that the band of 450 bp of primer OPB03 was linked to it at 15.4 cM. As OPB03450 is not close enough from the Co-5 gene of TU, it will not be useful during the pyramidation programs. However, this molecular marker can be useful for identification of the Co-5 allele in other cultivars that carry this gene.