RAPD marker assessment of self-pollinated inbreeding methods for common bean segregant populations
Ana Luiza Monteiro Castanheira and João Bosco dos Santos
RAPD markers were used to assess the potential five inbreeding methods have to release genetic variability. These methods were employed until family selection in the F5 generation, plus the parents Carioca and Flor de Mayo, and the test cultivar Pérola. DNA was extracted from 16 plants per family for RAPD reaction. Twenty-two primers amplified DNA fragments linked to a number of grain yield loci, weight of 100 seeds, number of days to flowering, and reaction to the oidium and angular leaf spot. To estimate genetic similarities between every genotype pair in each inbreeding method 42 polymorphic bands amplified among the families and parents were used. Genetic similarities were UPGMA-clustered and grouped by multidimensional scaling. The bulk method was most efficient to release genetic variability, followed by bulk in F2 families, SSD, bulk in F3 families, and pedigree. The bulk method created families with higher similarities close to the Carioca, which is one of the regionally most grown cultivars.