Targeting and genotyping RGAs in a mapping population of the AA genome of wild Arachis
Soraya Cristina de Macedo Leal-Bertioli, Patrícia Messenberg Guimarães, and David John Bertioli
Peanut is an important legume crop. Although it has high levels of morphological diversity, it lacks genetic variability and sources of disease resistance. The transference of resistance genes from wild species is difficult due to the different ploidy level of the wild and cultivated species. Recently, amphidiploids have been produced that can be used as bridges to introgress wild genes. Molecular markers are useful to pyramidize desirable genes and track them through generations of backcrossings. Molecular markers based on Resistance Gene Analogs have improved chances to be present in or linked to resistance gene loci. This study describes the development and genotyping of molecular markers based on resistance gene motifs. Specific primers were designed based on unique sequences of an Arachis RGA dataset. The identity of the amplified polymorphic bands was confirmed by sequencing. These markers were genotyped on a F2 population that segregates for resistance to biotic stress types.